SPR可以進行藥物靶量測量，從藥物-膜的相互作用，到藥物-細胞的相互作用。在實時和無標記的情況下區(qū)分內(nèi)部和滲透。研究細胞在不同涂層的附著情況。
基于細胞的研究有很多，包括組織工程、體外毒物學(xué)、藥物輸送，還有抗菌涂料。表面等離子體共振最初是用于蛋白質(zhì)相互作用測量的。直到近年來，研究人員才開始開發(fā)SPR來研究活細胞。
我們的一些用戶使用SPR來測量細胞在不同的生物材料和涂層表面的擴散或粘附。
SPR也可作為生物傳感器用于檢測細胞特定代謝物以確定細胞分化和細胞功能
一些小組正在研究生物膜的形成或抗菌涂料
五個選擇SPR檢查活細胞的原因
1. 能夠?qū)崟r測量活細胞和細菌附著
2. 能夠在未標記的情況下區(qū)分滲透和內(nèi)吞
3. 表面非特異性吸附情況
4. 溫度和剪切應(yīng)力的控制
5. SPR與電化學(xué)的結(jié)合
SPR可以回答的關(guān)于活細胞的五個關(guān)鍵性問題
1. 藥物吸收途徑是什么?
2. 哪一種納米顆粒是藥物輸送的最佳載體?
3. 一種納米粒子或病毒是如何進入細胞的?
4. 細胞附著在表面上的動力學(xué)是什么?
5. 哪一種表面最能抵抗細菌的生長?
參考文獻
1. Endothelial cells' biophysical, biochemical, and chromosomal aberrancies in high ‐ glucose condition within the diabetic range, Cell Biochemistry & Function, 2017, p. 1-15
2. Feasibility Study of the Permeability and Uptake of Mesoporous Silica Nanoparticles across the Blood-Brain Barrier, PLoS ONE 11(8): e0160705, 2016
3. Real-Time Label-Free Monitoring of Nanoparticle Cell Uptake, Small, 2016
4. Real-Time Protein and Cell Binding Measurements on Hydroxyapatite Coatings, J. of Functional Biomaterials, 2016, Vol. 7(3), p. 23
5. Biomimetic collagen I and IV double layer Langmuir–Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells, Biomaterials, Volume 1, May 2015, Pages 257-269
6. Structural and Viscoelastic Properties of Layer-by-Layer Extracellular Matrix (ECM) Nanofilms and Their Interactions with Living Cell, ACS Biomater. Sci. Engineering, 2015, 1[9]: p. 816–824
7. Microbial attachment and adsorption–desorption kinetic of tightly bound extracellular polymeric substances on model organic surfaces, Chemical Engineering Journal, 2015, 279: p. 516–521
8. Investigation of cell behaviors on thermo-responsive PNIPAM microgel films, Colloids and Surfaces B: Biointerfaces, 132: p. 202–207
9. Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility, J. of Biomed. Mat. Research Part A, 2013, 101A, 413–420
10. Oncolytic adenoviruses coated with MHC-I tumor epitopes increase the anti-tumor immunity and efficacy against melanoma, Capasso et al., Taylor & Francis Online, 2015
