| 型號(hào)/Model | SPR-T200 | SPR-T210 | SPR-I300 | |
| 光源 Light Source |
類型/Type | He-Ne Laser | He-Ne Laser | He-Ne Laser,and LED |
| 波長/wavelength | 632.8nm | 632.8nm | 632.8nm,and 621±10nm | |
| 轉(zhuǎn)臺(tái) Rotation Stage |
轉(zhuǎn)臺(tái)數(shù)量 Number of Rotation stage |
Motor 1, sample motor Motor 2, detector motor |
Motor 1, sample motor Motor 2, detector motor |
Motor 1, sample motor Motor 2, detector motor |
| 轉(zhuǎn)臺(tái)控制 Control of rotation stage |
軟件自動(dòng)控制 Automatically control |
軟件自動(dòng)控制 Automatically control |
軟件自動(dòng)控制 Automatically control |
|
| 轉(zhuǎn)臺(tái)角度精度 Rotation angular resolution |
<= 0.02° 可升級(jí)/upgradable |
0.01° 可升級(jí)/upgradable |
0.01° 可升級(jí)/upgradable |
|
| 轉(zhuǎn)臺(tái)旋轉(zhuǎn)角度范圍 Rotation angles |
0-360°(Motor 1) 0-180°(Motor 2) |
0-360°(Motor 1) 0-180°(Motor 2) |
0-360°(Motor 1) 0-180°(Motor 2) |
|
| 最佳測量角度范圍 Optimal measurable angles |
15-80°(incident angle) (for prism 1.845) |
15-80°(incident angle) (for prism 1.845) |
15-80°(incident angle) (for prism 1.845) |
|
| 轉(zhuǎn)臺(tái)防撞系統(tǒng) Anti-collision system |
兩個(gè)行程開關(guān) AZ-721， detector motor 超出 0-180°范圍時(shí)關(guān)閉電機(jī)2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. | 兩個(gè)行程開關(guān) AZ-721， detector motor 超出 0-180°范圍時(shí)關(guān)閉電機(jī)2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. | 兩個(gè)行程開關(guān) AZ-721， detector motor 超出 0-180°范圍時(shí)關(guān)閉電機(jī)2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. | |
| 角度校準(zhǔn) Angular calibration |
半自動(dòng) Semi-automatic |
半自動(dòng) Semi-automatic |
半自動(dòng) Semi-automatic |
|
| 載物臺(tái) Stage |
平臺(tái)控制類型 Motion Type |
手動(dòng) Manual |
手動(dòng) Manual |
手動(dòng) Manual |
| 移動(dòng)平臺(tái) Translation stage |
XYZ 移動(dòng)平臺(tái) | XYZ 移動(dòng)平臺(tái) | XYZ 移動(dòng)平臺(tái) | |
| 檢測器 Detector |
光檢測器 Photo detector |
光電二極管 Photo-diode detector |
光電二極管 Photo-diode detector |
光電二極管+CCD相機(jī) A photo-diode detector, and a CCD camera |
| 流通系統(tǒng) Flow Cell System |
進(jìn)樣系統(tǒng) Injection system |
蠕動(dòng)泵 Peristaltic Pump |
蠕動(dòng)泵 Peristaltic Pump |
蠕動(dòng)泵 Peristaltic Pump |
| 型號(hào) Type | BT100-2J | BT100-2J | BT100-2J | |
| 通道數(shù) Pump channels |
2 | 2 | 2 or 4(Optional) | |
| 進(jìn)樣控制 Injection Control |
自動(dòng)和手動(dòng)均可 Both Automatic and Manual |
自動(dòng)和手動(dòng)均可 Both Automatic and Manual |
自動(dòng)和手動(dòng)均可 Both Automatic and Manual |
|
| 進(jìn)樣體積 Injection Volume |
20-750 μl | 20-750 μl | 20-750 μl | |
| 流通池容量 Flow Cell Capacity |
20-100 μl | 20-100 μl | 20-100 μl | |
| 流通通道數(shù)量 Flow Cell Number |
單通道 Single Channel |
單通道 Single Channel |
1-4通道（可選） 1-4 Channels (Optional） |
|
| 基本參數(shù) Basic Parameters |
設(shè)備尺寸 Instrument Size |
1120 mm*560 mm*560 mm | 1120 mm*560 mm*500 mm | 1100 mm*560 mm*500 mm |
| 設(shè)備重量 Instrument Weight |
約120 kg | 約120 kg | 約120 kg | |
| 折射率靈敏度 Sensitivity |
10-5-10-6 RIU Depends on the sensor chips |
10-6 RIU Depends on the sensor chips |
10-6 RIU Depends on the sensor chips |
|
| 配件 Accessories |
棱鏡 Prism |
折射率/Refractive index, 1.745或1.845 可調(diào)換/Exchangeable |
折射率/Refractive index, 1.745或1.845 可調(diào)換/Exchangeable |
折射率/Refractive index, 1.745或1.845 可調(diào)換/Exchangeable |
| 流通池 Flow cell |
Quartz and PDMS | Quartz and PDMS | Quartz and PDMS | |
| 管 Tubes |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
|
| 匹配油 Matching Oil |
1.7000±0.0002 Cargille Labs |
1.7000±0.0002 Cargille Labs |
1.7000±0.0002 Cargille Labs |
|
| 樣品芯片 Sample Chips |
Sensor chips with 50 nm Au 可定制/Customizable |
Sensor chips with 50 nm Au 可定制/Customizable |
Sensor chips with 50 nm Au 可定制/Customizable |
|
| 其他 Others |
電源 Power Supply |
220V/50 HZ AC | 220V/50 HZ AC | 220V/50 HZ AC |
| 軟件 Software |
Sensby SPR | Sensby SPR | Sensby SPR | |
| 操作系統(tǒng) System |
Windows 7, 10 | Windows 7, 10 | Windows 7, 10 | |
| 蛋白質(zhì)組學(xué)研究（Proteomics） | 免疫測定法（Immunoassay） |
| 新藥研發(fā)（Drug Discovery） | 疫苗開發(fā)（Vaccine Development） |
| 癌癥研究（Cancer Research） | 瞬時(shí)結(jié)合（Transient Binding） |
| 信號(hào)傳遞 (Cell Signaling ) | 配體垂釣（Ligand Fishing） |
| 多分子復(fù)合物的結(jié)構(gòu)和組裝（ Multi-molecular Complexes） | 結(jié)合特異性（Binding Specificity） |
| 分子識(shí)別（Molecular Recognition） | 結(jié)構(gòu)與功能的關(guān)系（Structure-function Relationship） |
| 免疫調(diào)節(jié)（Immune Regulation） | 酶反應(yīng)（Enzyme Reaction） |
如圖所示，SPR 分子相互作用儀器的原理是波長 632.8 nm 的激光形成的 p 偏振光，到達(dá)棱鏡后激發(fā)金屬表面的表面等離子體發(fā)生共振。通過改變?nèi)肷涔饨嵌?θ，實(shí)時(shí)檢測反射光強(qiáng)度的變化 R，根據(jù)反射光強(qiáng)度變化 ΔR 或共振角度的變化 Δθ，實(shí)現(xiàn)分子相互作用的檢測；也可以通過固定角度，測量反射光強(qiáng)的變化曲線獲得分子相互作用動(dòng)力學(xué)常數(shù)。
| 型號(hào)/Model | SPR-T210 | SPR-I400 | |
| 檢測器 Detector |
光檢測器 Photo detector |
光電二極管 Photo-diode detector |
CCD CCD |
| 流通系統(tǒng) Flow Cell System |
進(jìn)樣系統(tǒng) Injection system |
蠕動(dòng)泵 Peristaltic Pump |
注射泵 Peristaltic Pump |
| 進(jìn)樣控制 Injection Control |
半自動(dòng) Semi-Automatic |
自動(dòng) Automatic |
|
| 樣品臺(tái) Sampling |
-- | 96孔板 自動(dòng)切換 |
|
| 進(jìn)樣體積 Injection Volume |
20-750 μl | 20-750 μl | |
| 流通池容量 Flow Cell Capacity |
20-100 μl | 20-100 μl | |
| 流通通道數(shù)量 Flow Cell Number |
單通道 Single Channel |
4通道 4 Channels |
|
| 基本參數(shù) Basic Parameters |
設(shè)備尺寸 Instrument Size |
1120 mm*560 mm*500 mm | 850 mm*630 mm*611 mm |
| 設(shè)備重量 Instrument Weight |
約120 kg | 約50 kg | |
| 樣品折射率測量范圍 Measurable Sample Refractive Index |
1~1.5 | 1~1.5 | |
| 折射率靈敏度 Sensitivity |
~10-6 RIU Depends on the sensor chips |
~10-6 RIU Depends on the sensor chips |
|
| 濃度定量靈敏度 Sensitivity for Concentration Detection |
10 ng/ml | 10 ng/ml | |
| 定量范圍 Detection Range |
0.1 μg/ml – 5 mg/ml | 0.1 μg/ml – 5 mg/ml | |
| 檢測濃度范圍 Detection Range |
0.05 nM-1 mM | 0.05 nM-1 mM | |
| 檢測解離常數(shù)（KD）范圍 KD Range |
0.05 nM-0.1 mM | 0.05 nM-0.1 mM | |
| 配件 Accessories |
棱鏡 Prism |
折射率/Refractive index, 1.845 可調(diào)換/Exchangeable |
折射率/Refractive index, 1.845 可調(diào)換/Exchangeable |
| 流通池 Flow cell |
Quartz and PDMS | Quartz and PDMS | |
| 管 Tubes |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
|
| 匹配油 Matching Oil |
1.7000±0.0002 Cargille Labs |
1.7000±0.0002 Cargille Labs |
|
| 樣品芯片 Sample Chips |
Sensor chips with 50 nm Au 可定制/Customizable |
Sensor chips with 50 nm Au 可定制/Customizable |
|
| 其他 Others |
電源 Power Supply |
220V/50 HZ AC | 220V/50 HZ AC |
| 軟件 Software |
Sensby SPR | Sensby SPR | |
| 操作系統(tǒng) System |
Windows 7, 10 | Windows 7, 10 | |
